「PCR method」に関連した動画の一覧

	Polymerase Chain Reaction (PCR)  The polymerase chain reaction, or PCR, amplifies a specific DNA fragment from a complex mixture. This video explains the process. This video is from: Essential Cell Biology, 3rd Edition Alberts, Bray, Hopkin, Johnson, Lewis, Raff, Roberts, & Walter ISBN: 978-0-8153-4129-1 2009年04月17日再生回数 216351
	PCR Polymerase chain reaction  Polymerase chain reaction The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified. PCR can be extensively modified to perform a wide array of genetic manipulations. Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the bacterium Thermus aquaticus. This DNA polymerase enzymatically assembles a new DNA strand from DNA building blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides (also called DNA primers), which are required for initiation of DNA synthesis. The vast majority of PCR methods use thermal cycling, ie, alternately heating and cooling the PCR sample to a defined series of temperature steps. These thermal cycling steps are ... 2011年01月14日再生回数 9090
	DNA Test Methods - The Polymerase Chain Reaction  View the full Interactive Tutorial at: www.phgfoundation.org The polymerase chain reaction, or PCR, is a crucial method for exponentially increasing the amount of a specific DNA sequence. PCR is a cyclic process controlled by the temperature of the reaction mixture. First, the temperature is raised to 95°C (205°F), causing the template DNA to separate (denature). The temperature is then decreased to around 50°C (122°F), allowing short primer pieces of DNA to hybridise to each strand of the template at opposite ends of the sequence to be amplified. The thermostable enzyme Taq (a DNA polymerase that is active at high temperatures) then binds to and extends the primers at an intermediate temperature of 72°C (162°F), so that two new double-stranded copies of the template are made. This cyclic process of separating DNA strands, copying and reannealing the daughter strands is repeated multiple times to increase the numbers of DNA product. 2008年10月08日再生回数 19089
	6 Quantitative PCR -- the deltadeltaCt method  How can I correct a qPCR result by internal controls? The delta delta Ct calculation, and its application in the real world. 2011年09月10日再生回数 2984
	PCR - DNA Fingerprinting  Free Science Help at Brightstorm! brightstorm.com The PCR method of DNA Fingerprinting. 2010年09月02日再生回数 26714
	3_Quantitative PCR - the deltaCt method  How can you calculate the ratio of templates by setting a threshold of fluorescence intensity, measuring the number of PCR cycles required to reach it? 2011年09月10日再生回数 1563
	Real-Time Polymerase Chain Reaction (PCR)  This short animation introduces the real-time polymerase chain reaction (PCR) procedure. This resource was developed by Yaw Adu-Sarkodie of the Kwame Nkrumah University of Science and Technology and Cary Engleberg of the University of Michigan. It is part of a larger learning module about laboratory methods for clinical microbiology (available at open.umich.edu 2009-2010, Kwame Nkrumah University of Science and Technology and Cary Engleberg. This is licensed under a Creative Commons Attribution Noncommercial 3.0 License creativecommons.org 2010年12月24日再生回数 92769
	LAMP PCR Lights the Way for a Simple, Fast Method for Detection of Lso  2011 Conference presentation by Dr. Dennis Gross and Aravind Ravindran 2011年12月20日再生回数 254
	PCR Cloning  ( www.abnova.com ) - PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA, (3) ligation into a plasmid vector, and (4) transformation into bacteria and then bacteria will replicate the plasmid. More videos at Abnova www.abnova.com 2010年03月31日再生回数 11126
	KIR typing by PCR-SSP: tips for critical steps  This video shows two critical steps in the process of KIR-gene typing by PCR-SSP: 1-How to prepare 24 ready-to-use typing sets 2-The rapid loading of six typing assays into an agarose gel, with the help of an 8-channel pipetting device. Further technical details published in: 1.-Vilches et al. Tissue Antigens 2007, 70:415-422. DOI: 10.1111/j.1399-0039.2007.00923.x 2.-Ordóñez et al. In Immunogenetics: Methods and Protocols (eds.: FT Christiansen and BD Tait). Methods in Molecular Biology. Humana Press (in process of publication, 2011) contact: camycvilches-video@yahoo.com 2011年02月09日再生回数 638